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The RNA was then treated with RapidOut DNA Removal Kit (K2981, Thermo) according to the user guide to remove trace genomic DNA residue

doi: 10.1038/ncomms11457 68 ZhouQLiLShaFLeiYTianXChenLet al

In atopic dermatitis, keratinocytes from the compromised skin barrier release cytokines such as thymic stromal lymphopoietin (TSLP), IL-25, and IL-33, which activate APCs to express OX40L [47]