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Moreover, unlike many current predicting tools that work at a fixed collision energy 21,23,43 , ESI-MS/MS spectra predicted using DeepCDM are collision-energy-merged, which is more compatible with experimental conditions acquired by LC-MS based non-targeted analysis 27,28,29,30,31,32

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Quantitative real-time PCR mRNA was extracted using the Cells-to-Ct kit (Ambion)