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0.4 M recombinant human His-sumo-GSDME was preincubated with 100 M 829 compounds from In-house Human Endogenous Metabolite Compound Library dispensed into 96-well plates for 0.5 h before addition of 0.2 M recombinant active-caspase-3 for another 2 h at 37 C

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Then, taking advantage of the recently reconstructed genome-scale metabolic model iSM454 for O

NADPH and inducible NO synthase produce superoxide anion (O 2 ) and NO after stroke, which in turn produce peroxynitrite ion (ONOO ) and further produces factors that increase the activity of MMPs (Chen et al., 2016), which destroy tight junctions and the BBB (Gu et al., 2011)