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Gene analysis Reverse transcription and total RNA extraction of the target genes Total RNA was obtained from ovary samples using the Trizol reagent in accordance with the manufacturers instructions (Direct-zol TM RNA MiniPrep, cat No

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The necropsy was performed following protocols established within the Retrovirus Laboratory of the Department of Molecular and Comparative Pathobiology at Johns Hopkins Medicine, in accordance with the American Veterinary Medical Association guidelines for euthanasia of animals 37, 38, 53, 54

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