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However, a notable side effect was observed: MT-II-treated normal C57BL/6J mice exhibited significant weight loss, with their average weight decreasing from a baseline of 30.9 1.4 grams to 26.5 1.7 grams after the 14-day treatment course, a change not seen in vehicle-treated controls

Throughout the study, safety assessments included physical examinations, vital sign measurements, electrocardiograms, laboratory testing, adverse event monitoring, and the administration of the Beck Scale for Suicidal Ideation

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This structured approach improves both safety and consistency