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coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)

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Epub 2019 May 31

doi: 10.3389/fendo.2022.1049665 78 JlvingLRNielsenJKesmodelUSNielsenRNorgardBMBeck-NielsenSS

[10] Specifically, the time taken for the wounds to fill was reduced from 25 days in controls to 14 days in the GHK group

NAMPT and NRK1/2 are also localized to the nucleus and cytosol 48,83,84 , suggesting that only the final step of salvage is performed in the mitochondria, with a mitochondrial NMN transporter, Slc25a45, recently proposed 85