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resultant supernatant fractions were centrifuged at 1,000 g for another 15 min, followed by centrifugation of the supernatant at 3,000 g for 15 min

In the longer run gives lighter, brighter skin tone to lighten and also helps decrease wrinkles and increase skin elasticity

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The constructs were validated by DNA sequencing

VITAMIN C: A potent antioxidant that inhibits melanin production, reduces the appearance of dark spots, and stimulates collagen synthesis for firmer, more youthful skin

Cells were washed with cold PBS and then fixed with 70 % cold ethanol for 15 min on ice