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To differentiate ESCs into neural progenitor cells (NPCs), we used a variation of the dual SMAD inhibition protocol 44,45,46,47,48,49

To finish, I would like to point readers also to the wide diversity of ETCs across living systems introduced briefly with further reading in [1], and to the surge in medical research publications reporting measurement of bioenergetics parameters in mammalian cells

However, another study using a comparable model reported elevated cytokine and oxidative stress markers in the cortex and hippocampus without detectable changes in neurogenesis ( 6 Mechanisms of chronic stress-induced neuronal dysfunction 6.1 Neuroinflammation Neuroinflammation refers to immune activation and inflammatory responses within the central nervous system (CNS), typically involving sustained activation of microglia and astrocytes as well as excessive release of proinflammatory cytokines (Sotzny et al., 2018), increased numbers of activated CD8 + cytotoxic T cells (Rivas et al., 2018), and elevated levels of multiple proinflammatory cytokines (Montoya et al., 2017)

doi: 10.1073/pnas.1005225107 88 ZhaoH