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Description

PloS One (2020) 15:e0233208

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Glutathione Metabolism Ferroptosis can be induced by compounds interfering with system x c , such as erastin, which induces cysteine deprivation, GSH depletion, endoplasmic reticulum stress, and cell death (Sato et al., 2018)

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Percentages of proliferative (BrdU + ) (n = 6 technical replicates representative of 2 independent experiments) ( a ) and apoptotic (Annexin V + ) (n = 3 technical replicates representative of 3 independent experiments, **p = 0.0042) ( b ) cells were determined

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Methods: iPSCs were differentiated to iPSC-Heps over 22 days

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Also, higher levels of glutathione are known to preserve telomeres, which are the tail ends of genes that dictate how many times the cell can regenerate

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After washing and resuspension, the sample tubes were analyzed by flow cytometry by BD FACSymphony

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