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In a total of 20 studies (14 observational studies, 1 intervention study, and 5 rodent studies) for the systematic evaluation and analysis of VD and PD, it was reported in a meta-analysis of six observational studies reporting differences in VD levels that mean serum 25(OH)D levels in PD patients were lower than those in healthy controls, five studies in animal models of PD showed that supplemental VD intake in rodents significantly increased the concentrations of tyrosine hydroxylase, promoted the conversion of dopamine neurotransmitter, significantly decreased oxidative damage to the substantia nigra, and significantly increased dopamine levels and neuronal survival in the substantia nigra (87)

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After 24 h of incubation, spheroids were collected from the microwells using cut 1000 L pipette tip and transferred into a sterile 10-cm Petri dishes in cortical differentiation medium [E6F with 10 M dorsomorphin (DM

& Chavarro, J

Int J Mol Sci 22(5):2596