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Description

Ascorbate peroxidase in tea leaves: occurrence of two isozymes and differences in their enzymatic and molecular properties

Channels (Austin) 15(1):483495

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After treatment, tissues were maintained in 2% FBS DMEM until the 10th days, the medium was changed every 3 days

Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 570 nm Precision pipettes to deliver 2 L to 1 mL volumes Tubes to prepare sample dilutions Incubator at 37C 50 mL conical tubes Deionized water Assay Procedure Summary Prepare the Reaction Mix (+LGOX), and Background Mix (-LGOX) before use