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Cell culture and viability assay Raw 264.7 cells, originating from mouse macrophages, were maintained in a complete medium consisting of 90% high-glucose Dulbeccos Modified Eagle Medium (DMEM) and 10% fetal bovine serum (FBS), at 37 C in a 5% CO 2 incubator

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Morphine hyperalgesia gated through microglia-mediated disruption of neuronal Cl(-) homeostasis

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A growing body of scientific evidence on mammalian systems indicates that AITC treatment arrests the cell cycle, causes apoptosis and inhibits the proliferation of cancer cells leading to reduced tumour formation in certain types of cancer 14,15