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J Neurosci 30(5):16311636 Arciello M, Rotilio G, Rossi L (2005) Copper-dependent toxicity in SH-SY5Y neuroblastoma cells involves mitochondrial damage

Symptoms of vitamin B12 deficiency may include any or all of the following: Chronic fatigue (4) or low energy (despite getting enough sleep) Shortness of Breath Macrocytic anemia (5) High MCV (mean corpuscular volume on CBC analysis) Neurological changes that can mimic dementia (6) Depression (7), anxiety, and other mood disorders Difficulty with concentration (8) Brain fog Skin changes like hyperpigmentation (9), vitiligo, or angular stomatitis Hair changes (including hair loss (10)) Perhaps you can relate to one or more of these symptoms noted above

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For MGMT staining, slides were blocked with 5% goat serum in PBS for 1 h at room temperature (RT), and subsequently incubated with primary antibody (5 g/ml rabbit anti-MGMT, PA5-79668, ThermoFisher Scientific, Germany), for 1 h at RT

Adenosylcobalamin Adenosylcobalamin is also a naturally occurring, co-enzyme form of Vitamin B12

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