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The column temperature was 30 C, and the mobile phase was a mixture of A [50 mM phosphate buffer (pH 3.0) containing 10 mM sodium 1-heptanesulfonate] and B (methanol) with an A/B ratio of 95/5 at a flow rate of 1 mL/min

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To minimize potential sex differences subjects were randomized into treatment and placebo groups, despite randomization there was still an imbalance in males and females in each group

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