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Why Storage Rules Exist: The Chemistry Behind Stability Peptide degradation in solution occurs through three primary chemical pathways: hydrolysis (water cleaves the peptide backbone, most rapid at elevated temperature and acidic or alkaline pH), oxidation (methionine, cysteine, and tryptophan side chains react with dissolved oxygen or photocatalytic radicals), and deamidation (asparagine and glutamine residues lose an amide group, producing aspartate or glutamate and altering the peptide's charge and receptor binding)

doi: 10.1016/j.ccell.2021.05.002

eLife 2 , e01749 (2013)

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The connection is not fully understood, but several potential mechanisms exist

By following clear rules and tracking results, researchers can unlock the full potential of this peptide stack