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Early life microbiota colonization programs nociceptor sensitivity by regulating NGF production in mast cells

Fecal genomic DNA was extracted using the QIAmp Power Fecal DNA Kit (QIAGEN, Netherlands) according to the manufacturers instructions and quantified using the NanoDrop ND-1000 spectrophotometer (Thermo Fischer Scientific)

Here, we found mesencephalic astrocyte-derived neurotrophic factor (MANF), a hepatoprotective factor, was exceptionally upregulated in human ICC tissues and experimental mouse ICC models induced by sleeping beauty transposon (SBT) or thioacetamide (TAA) challenge

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GH pulse frequency and amplitude remained consistent across the observation window, suggesting that GHS-R1a undergoes limited downregulation under the studied exposure conditions in that species