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Description

Maybe I would have passed out from exhaustion the following day and been medevaced had I not been eliminated

The cell pellet was resuspended in lysis buffer A 1 (25 mM HEPES, pH 7.5, 500 mM NaCl, 1 mM Tris (2-carboxyethyl) phosphine (TCEP)) supplemented with EDTA-free Protease Inhibitor Cocktail (Selleck)