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Clean the injection site with an alcohol swab and allow it to dry completely

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Bacterial cells were harvested by carefully removing the supernatant by centrifugation (1,000 g , 10 min, 4 C) and the resulting pellet was resuspended with 50 l of B-PER reagent (supplemented with 1 mM DTT and 1 mM PMSF)

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Additionally, fouling and biofouling is a major concern in the treatment of cooling tower blowdown

The most frequently reported incidents are classified as swelling, redness, bumps, and blisters on or around the treated area