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Hereafter, inserts were washed twice with DMEM-F12 before the cells were added

13 Transplanted cells in the IVD face numerous challenges due to the harsh microenvironment

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95 explored the possibility that mtROS are O 2 sensors during hypoxia

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The different immune functions performed by DCs, as well as the transition from an immature to a mature state in response to stimulus signals, are modulated by multiple factors, such as selective gene expression, cytokines stimulation, antigen presentation, nutrient metabolism and so on ( Figure 1 The role of amino acids in protein synthesis is well-defined, and a large number of studies in recent years have demonstrated that amino acid metabolism has a profound effect on various types of immune cells, including bone marrow cells and lymphocytes ( in vitro using serum-free culture medium consistent with the average concentration of the plasma amino acid from a healthy volunteer (HCM) and that from patients with ACM and compared DCs function and glucose-amino acid metabolism in each medium