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All samples were pre-incubated for 30 minutes and further dialyzed for 4 h so that the final concentration of SF was 34 nM

doi: 10.3389/fphar.2023.1239395 Received 13 June 2023 Accepted 28 July 2023 Published 04 August 2023 Volume 14 - 2023 Edited by Xiang Zhou, Fudan University, China Reviewed by Ben Woolbright, University of Kansas Medical Center, United States Pranav Shah, National Eye Institute (NIH), United States Updates Copyright 2023 Li, Ni and Chen

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Furthermore, local targeting of NAD + salvage pathway by microparticle-mediated intratumoral delivery of NAMPT inhibitor GMX1778 induced striking immunologic changes in the tumor microenvironment of murine glioblastoma, characterized by upregulation of immune checkpoint PD-L1, recruitment of cytotoxic T cells, and reduction of tumor-associated macrophages, and potentiated checkpoint immunotherapy [114]

For most protocols, 100200mg/mL is the optimal concentration range