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In most organisms studied to date, these processes predominantly occur in the cytosol or in plastids of plants (Kruger and von Schaewen, 2003

Similar content being viewed by others Introduction The classical way to generate temperature-sensitive alleles comprises random mutagenesis followed by large-scale screens at different temperatures

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To facilitate exchange of deuterated amides back to protons, the purified protein was incubated with 2.5 M guanidinium-HCl and 5 mM EDTA for 3 h, then dialyzed into NMR buffer (10 mM sodium phosphate, pH 7.0, 150 mM NaCl)