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The column was washed with 6 bed volumes of wash buffer (50 mM sodium phosphate, 300 mM NaCl and 100 mM imidazole, pH 7.4) and the target protein was eluted with 6 bed volumes of elution buffer (50 mM sodium phosphate, 300 mM NaCl and 300 mM imidazole, pH 7.4)

The anti-asthmatic potential of flavonol kaempferol in an experimental model of allergic airway inflammation

Ising C, Venegas C, Zhang S, Scheiblich H, Schmidt SV, Vieira-Saecker A, Schwartz S, Albasset S, McManus RM, Tejera D, Griep A (2019) NLRP3 inflammasome activation drives tau pathology

Autism Res 8(2):224228

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The injections are generally well tolerated