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J Neurochem 73:14221430 Had-Aissouni L (2011) Toward a new role for plasma membrane sodium-dependent glutamate transporters of astrocytes: maintenance of antioxidant defenses beyond extracellular glutamate clearance

10.1371/journal.pone.0133896 PLoS One 266 YinR.MaoS

Cell grouping and treatment Cigarette smoke extract (CSE) was prepared by bubbling the smoke from two cigarettes into 20 mL of serum-free DMEM, which was then filtered with a 0.2 m filter to sterilize the mixture

The most significant risk factors for advanced fibrosis in both sexes were obesity (BMI), abdominal obesity (waist circumference and waist-hip ratio), insulin resistance (HOMA-IR), and elevated transaminases (p 40 IU (p 30g/day), and mixed MAFLD (MAFLD and alcohol-related cirrhosis)

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This is largely due to the prevalence of genetic variations in the MTHFR (methylenetetrahydrofolate reductase) gene