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After subsequent washing, 50l/well of 1:1000 diluted streptavidin-ALP (Mabtech) was added, incubated for 1 hour, and then developed with 50 l/well of AP Conjugate sub kit (BioRad) for 510 minutes

Refresh Rate: 60 Hz

This conjugate explains the free vitamin B12 in the small intestine, and finally binds to a complex with an endogenous glycoprotein secreted with intestinal mucosa cells, absorbed from the end of the intestinal end

therefore, Lyme antibody titers should be performed if the patient's history suggests possible exposure