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The excess mortality reported in patients with CKD and high ALP levels has been controversially attributed to potential cardiovascular adverse effects as a result of very severe lack of control over bone-mineral metabolism.2,3,13,14 Several pathogenic mechanisms have been proposed to explain this association, including increased vascular calcification via pyrophosphate hydrolysis in the arterial wall,15 systemic inflammation16,17 and vitamin D deficiency.18 However, the absence of a significant association between serum levels of the bone-derived isoenzyme of ALP (bone-specific ALP) and mortality in patients with CKD19 raises serious doubts about the hypothesis on a pathogenic link with bone-mineral metabolism

Thus, a mixture of these compounds was used in a single round of negative screening, while hydroxytyrosol was used in the positive screening

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doi: 10.1002/jsfa.10184 175 XuM.LuY.YangH.HeJ.HuZ.HuX.et al