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[ Treatment of human umbilical vein endothelial cells (HUVECs) with UA triggered inflammatory response mediated by significant elevation of expression levels of IL-1, IL-6 and ICAM

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The resultant cell suspension (10 6 cells/mL) was loaded in a poly-L-lysine-coated 96-well culture plate and incubated at 37 C with 5% CO 2 overnight to allow for monocyte adherence