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66 TorresS.BalboaE.ZanlungoS.EnrichC.Garcia-RuizC.Fernandez-ChecaJ

fPSPs were monitored for 60 min post HFS, and data points were obtained by averaging six responses every minute

The purity of the neutrophil population was typically greater than 90%, as determined by morphological examination with Giemsa staining and flow cytometry, based on CD11b + (1:500, clone M1/70) and Ly6G + (1:500, clone 1A8) surface markers

Co-infection with BRSV and Mannheimia haemolytica triggered an exacerbated IL-17 response, which drove dysregulated neutrophil recruitment and perpetuated extensive immunopathological injury to the lung parenchyma 102