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The exact reasons for this have not been fully established but been suggested to be the effect of Arg-112 making ApoE4 in a tighter locked conformation than ApoE3 or ApoE2, thus hindering the lipid insertion site (Chen et al., 2021

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You can find genetic testing with various companies such as 23andme.com Its estimated that those with one mutation (heterozygous) decrease their ability to methylate by 30% and those with two mutations (homozygous) decrease their ability to methylate by 70%, making it virtually impossible to get adequate B12 from diet alone (19)

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