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Furthermore, four ubiquitin fragment peptides N20 (residues 1-20), M1 (residues 21-40), M2 (residues 39-58), and C19 (residues 58-76) were synthesized and tested for Ufd1 binding ( Extended Data Fig

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The resultant 11-hydroperoxyeicosatrienoic acid (11-HPETE) spontaneously degrades to 11-hydroperoxydienoic acid (11-HPODE) and the (Z)-non-3-enal, which is then catalytically converted to 4HNE by cytochrome p450 isozyme CYP2E6 (and others)