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Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100

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modulator=DFO, modulator concentration=100 M, lethal=Erastin) the modulatory effect (M e ) was equivalent to the maximum observed deviation, positive or negative, from B Exp

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many key links (such as the direct regulation of iron uptake molecules by androgens) still rely on indirect evidence or inference and urgently require experimental validation