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In controlled laboratory models, researchers use GHK-Cu to investigate extracellular matrix (ECM) remodeling, copper balance in cells, and redox signaling (how cells respond to oxidative stress)

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For post-PCR cleanup, DNA clean beads were added in a volume 1.5 times the volume of DNA

R162A and S353Y double mutant) were used to replace the EGFP gene, generating the AAV-hHPN WT and AAV-hHPN RS vectors

The effect of KD intervention on the brain is mediated indirectly by short-chain fatty acids (SCFAs), which are produced by Akkermansia and Lactobacillus [123, 124], and transported by monocarboxylate transporters expressed on the astrocytes of blood-brain barrier (BBB)

Induction of antioxidant enzymes by ARE is mediated by Nrf2