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To introduce the C-terminal Azo-tag into the coding region for the sfGFP reporter protein, two Gly codons and the amber stop codon were inserted between the C-terminal Strep -tag II and the final ochre stop codon, thus placing Pap as the last encoded residue in the reading frame, and this gene cassette was subcloned on pSB19 between the Xba I and Hin dIII restriction sites

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Experimental Procedures Human Induced Pluripotent Cell Culture All pluripotent and reprogramming cell cultures were maintained at 37 C in Heracell VIOS 160i direct heat humidified incubators (Thermo Scientific) with 5% CO 2 and 5% O 2

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This selectivity is why ipamorelin became the preferred GHRP in anti-aging and performance research

Rodbell, M