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If there is presence of enzymatic activity and/or spontaneous oxidation in storage, we would expect to observe lower levels of total GSH and free GSH, and elevated levels of GSSG, in the EDTA-treated sample stored at 4 o C compared to the non-deproteinized EDTA-treated plasma stored at 80 o C

10.25259/jrhm_31_2024 26 Di BerardinoC.PesericoA.CapacchiettiG.ZappacostaA.BernabN.RussoV.et al (2022)

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Inclusion of patients with preexisting liver disease who are receiving low-dose APAP is also a comparison group for which little data exist

(PubMed) Zeisel SH, Warrier M

Thus, the history of vaccination and infection of participants should be included in the analysis to minimize this source of variation [54,62]