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Those with higher ergothioneine levels showed: 50% reduced risk of cognitive decline over five years Better memory performance across all age groups Brain activity patterns more similar to younger individuals on cognitive tasks Professor Barry Halliwell, one of the worlds leading experts on antioxidants at the National University of Singapore, explained the mechanism: Ergothioneine gets into mitochondria and preserves mitochondrial function in disease

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While the sample was not large, (five in the treatment group and five in the control group) this study found a significant reduction in DSBs in the treatment group compared to controls [56]

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The fluorescence intensity of ROS was quantified using ImageJ 1.4 (National Institutes of Health, Bethesda, MD) and expressed in arbitrary fluorescence intensity units (A.U.)