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The gels were incubated in a renaturation buffer for 60 min at room temperature, followed by incubation for 18 h in 100 ml of developing buffer at 37 C under gentle shaking

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10.1155/2015/363801 289 TusiimireJ.WallaceJ.WoodsN.DuftonM

By calming the bodys inflammatory response, this treatment helps alleviate discomfort, improve mobility, and restore overall comfort

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