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Generation of XKR8-deficient HL-60 cells XKR8-deficient HL-60 cells were generated using CRISPRCas9 technology

coli , cells from 10 mL culture were resuspended in 1 mL of Buffer A (150 mM NaCl, 10%(v/v) glycerol, 0.1 mM EDTA, 0.2 mM TCEP, 0.2 protease inhibitor cocktail, 50 mM Tris-HCl pH 8.0) for sonication using an ultrasonic homogenizer (Cat

Nature 349:760765 Matute C, Domercq M, Fogarty DJ, Pascual de Zulueta M, Sanchez-Gomez MV (1999) On how altered glutamate homeostasis may contribute to demyelinating diseases of the CNS

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2020a;21:298308