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Typically the assay contains 60 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM dithiothreitol, 150 nM ARS enzyme, and a range of amino acid, ATP, and tRNA concentrations

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Conclusion Although changes in glutamate levels in schizophrenia have been linked to impaired glutamatergic neurotransmission and hypofunction of NMDA receptors, it is likely that glutamate alterations also result from metabolic impairments, particularly at mitochondrial level