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P.PeresR

Hemmings HC, Egan TD, eds

When the cellular activity promoting ferroptosis significantly exceeds the antioxidant buffering capacity provided by the ferroptosis defense system, there is an excessive accumulation of lipid peroxides on the cell membrane and subsequent membrane rupture, leading to cellular ferroptosis (Stockwell et al., 2020

The beads were washed 4 times with ice-cold RIPA buffer (50 mM HEPES, pH = 7.5, 500 mM LiCl, 1 mM EDTA, 1.0% NP-40, 0.7% Na-Deoxycholate), followed by once with ice-cold TE buffer (with 50 mM NaCl)