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A comprehensive review was conducted of PubMed citations containing the key word marker OR biomarker combined with migraine OR headache. Other key words included serum, saliva, cerebrospinal fluid, genes, blood, and inflammation. The only restriction was English-language publication

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Secondary endpoints were defined as pharmacodynamics and pharmacokinetic parameters including maximum serum concentration, time to maximum concentration, half-life, plasma clearance, and volume of distribution, as well as exploratory parameters including bodyweight, glycemic control, and overall hormone profile

However, most people excrete excess B12 harmlessly, and hair loss from overdose is uncommon

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More water means a more dilute solution, which means larger volumes per dose but potentially more precision for smaller doses