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Description

Following treatment, media was replaced with 80% (v/v) methanol (cooled to 80 C.) containing 500 nmol 6-chloronicotinamide (6-CN) as an internal standard (IS) to extract cellular metabolites

If a hydrophobic peptide is cloudy in neutral water but turns transparent after adding 0.6% acetic acid, the original issue was solvent choice, not purity