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Eur J Heart Fail 16(7):772777 Karimi Galougahi K et al (2015) Redox biomarkers in cardiovascular medicine

Wash with PBS one more time and add 500 l of lysis buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5) on ice

By influencing metabolic reprogramming, itaconate can suppress proinflammatory Th17 responses while enhancing Treg differentiation, thereby restoring the Th17/Treg balance critical for controlling autoimmune inflammation (94)