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Cycle sequencing was performed on a 3130 Genetic Analyzer using the BigDye Terminator v3.1 kit (Life Technologies)

These metabolic changes align with significant disruptions in amino acid metabolism, along with disturbances in glutathione biosynthesis and spermidine/spermine metabolism in male patients with PARK2 , PINK1 , or PARK7 mutations, as indicated by pathway enrichment analysis

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The toxicity of simulated microgravity on nematodes could be further assessed more recently 24,25,26,27