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Five hundred l of culture supernatants obtained after centrifugation for 10 min at 5000 g were mixed with an equal volume of PBS containing 1.12 mM phenol red (Sigma) and 19 U horseradish peroxidase (Sigma)

In deeply anesthetized rats, laparatomized before sacrifice, we assessed the gross lesions in the gastrointestinal tract and in the stomach (sum of the longest diameters, mm) (Gojkovic et al., 2020