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The supernatants were transferred to fresh tubes and the remaining peptides were sequentially extracted by incubating the gel pieces with 50% acetonitrile in 25 mM ammonium bicarbonate, 50% acetonitrile in 0.5% trifluoroacetic acid and then 70% acetonitrile in 0.5% trifluoroacetic acid

We also provide evidence for a positive feed-forward loop whereby SIRT1 up-regulates NQO1 level

L., Zarlenga, D

Liu X, Liu S, Tang Y, Pu Z, Xiao H, Gao J, et al

Wu T, Ding L, Andoh V, Zhang J, Chen L (2023) The mechanism of hyperglycemia-induced renal cell injury in diabetic nephropathy disease: an update