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Consult a dermatologist if you have specific skin conditions or are unsure about suitability

The supernatant was transferred to a new tube

C., Kanter, R

Cell culture and drug treatment The human liver cancer cell line HepG2 was obtained from ATCC and was cultured in DMEM glutamax high glucose medium (Invitrogen) supplemented with 10% fetal bovine serum and 100 U/mL penicillin/streptomycin (Invitrogen), in a humidified incubator at 37C and 5% CO 2 atmosphere

Quantitative PCR was performed using the Roche SYBER Green reagent and a LightCycler 480 apparatus (Roche Diagnostic, Meylan, France) with the following program: one step at 95C for 10 min, 40 cycles of denaturation at 95C for 10 sec, annealing at 65C for 15 sec and elongation at 72C for 15 sec

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