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An essential part of the RNA-induced silencing complex (RISC), which is part of the machinery that processes miRNA, is argonaute 2 (AGO2) [217], and the gene Drosha, which is in charge of digesting Pri-miRNA [218]

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The time points were at 1 min ligation-time (early effect) or at 15 min, 24 h or 48 h ligation-time (delayed application)

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