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For testing the difference in mean and median lifespan between groups, we used linear mixed model with linear quantile mixed-effects model, which included cage assignment as a random effect and batch as a covariate, respectively, using package nlme and lgmm in statistical software R

Individuals seeking weight loss should be directed towards evidence-based interventions including dietary modification, increased physical activity, and behavioural support

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It is important to note that whilst B complex supplements are widely available over the counter, they are not a substitute for a balanced diet

It contributes to the metabolism of carbohydrates and fats, playing a role in energy production

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