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Hepatic steatosis was further analysed by haematoxylin-eosin (H&E) histological staining (Fig

Cells were washed three times with HBSS buffer and permeabilized with 0.2% Triton X-100 for 15 min

The researchers found significantly elevated levels of superoxide dismutase (SOD), catalase, and malondialdehyde (MDA) ( 2.5 Changes in the vascular system (both blood and lymphatics) It is theorized that vascular dysfunction leads to excessive interstitial fluid accumulation in lipedema tissue, promoting adipose tissue expansion ( Histological observations suggest that the blood vasculature in lipedema tissue may be morphologically distinct from healthy controls, characterized by higher vessel density, as well as significantly dilated vessels ( in vitro evidence

Trends Mol Med 26:820

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Impaired endothelium-dependent vasodilation in patients with essential hypertension