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After 48 h, cells were stimulated with 50 ng/mM PMA and 1 g/mL ionomycin, followed by 3 h incubation with GolgiStop to evaluate intracellular cytokine secretion

Wei Q, Cheng L, Hong WK, Spitz MR

Figure 5 Taken together, these results indicate the treatment of sagittal brain slice cultures with 0.5 mg/mL LPC induces demyelination 24 h post-treatment that remains up to 5 weeks post-treatment

So I would just say spend more time on that conversation

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