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Dairy cows fed 3 mg Se/d in the form of Se-yeast had a greater increase in Se concentration in whole blood and had numerically elevated Se concentrations in skeletal muscle, heart, and liver compared to those fed 3 mg Se/d in the form of sodium selenite ( One of Se's most well-known biological roles is as a constituent of several glutathione peroxidase (GPx) isozymes which have an array of functions, including protecting against oxidative stress, minimizing inflammation, and regulating cell death

Tissue sections were then blocked with 5% bovine serum albumin (BSA) for 1 h and incubated overnight at 4 C with primary antibodies to -SMA and collagen I (Servicebio)